On the Tip of the Butterfly’s Tongue

Written by Nancy Miorelli

question

Puddling

The short of this answer is that butterflies have a behavior where they sit on river banks, dead stuff, or poop and lap up the salts and minerals. It’s a behavior that can be seen in a few other insects like bees and flies, however is most  apparent in butterflies.

Let’s take a further look into how and why butterflies puddle.

Butterfly Mouths 101

Before we jump right into things, we need to first take a look at the butterfly mouthpart bits. Most of the butterfly mouth is reduced and the parts don’t have a function anymore  (in most butterflies). However, two parts, the maxillary galeae fused to form the butterfly tongue which is called the proboscis. It acts basically like a straw.  There´s another part called the legulae which is part of the labium and has a top (dorsal) and bottom (ventral). This is where the action happens

General butterfly mouthparts. Well, the ones that are important for this article.

General butterfly mouthparts. Well, the ones that are important for this article.
Also, drawn on the back of a placemat in Ecuador.

Now that we’ve made it through the vocabulary, I can show you some cool pictures. It was previously thought that butterflies could only drink liquid due to their straw-like mouth parts. This gave rise to the question, how can butterflies drink viscous material and lap up salts from the sides of rivers?

The answer is with sponges. Little sponges. Microscopic sponges.

Microsponges

Remember all those fancy words a few paragraphs up? Well, now here is where we’re going to talk about them some more. The proboscis is hollow inside. The two fused galeae make a channel called the ¨food canal¨since that’s where the food gets sucked into. At the tip of the proboscis lie the legulae. The dorsal legulae (the one on top)  forms overlapping plates where as the ventral legulae (the ones on the bottom) form tight interlocking hooks.

These legulae  form the sponge at the tip of the butterfly’s tongue. The liquid sticks and pools in the sponge (legulae) and then is sucked up in the food canal (galeae) by capillary action.

Monaenkova et al. 2011

Monaenkova et al. 2011

The butterfly proboscis has been around for quite some time with this sponge. In fact, it’s been here since the Jurassic period but we really see a diversification in butterfly species during the Cretaceous  (146MYA). This was at the same time that plants were diversifying Therefore, we think as the plants  developed different types of flowers and fruits, butterflies (and moths) diversified to eat a wide range of foods. This includes rotting fruit. The sponge at the end of the proboscis allowed the butterflies to access more resources. The same sponge that opened up the rotting fruit option allowed butterflies to absorb nutrients from the soil.

Painting the Picture

Butterflies actually eat many different things. We tend to think of them as only as nectar feeders but butterflies will eat pollen, fruit, sweat, tears, and blood. Each of their microsponges have slightly different shapes based on what  they eat.

butterfly tongues

Butterfly tongue shape based on what the butterflies eat
Krenn, 2010

In fact, there are several non flower feeding butterflies that solely feed on fruit. These butterflies have lots of little hairs at the end of their tongue that appear to form a flat brush. The butterflies sweep the surface with their tongue and will dab wetter surfaces. The butterflies also will spit onto their food if it’s not wet enough to eat.

Why Puddle?

Isn’t this one of the questions of the century.

While most species of butterflies puddle, it’s usually only the males that we see puddling. Therefore, we naturally assumed that the males needed some extra nutrition to give to the females during mating.  Male insects deposit a sperm packet inside the female. This sperm packet includes sperm but also has essential amino acids, salts, and proteins.

So we’re assuming the butterflies are looking one or two things.  Sodium (salt) and nitrogen (to build proteins).

Puddling butterfly in the Maquipucuna Ecolodge in Ecuador.

Puddling Orange Daggerwing (Marpesia berania) in the Maquipucuna Ecolodge in Ecuador.
PC: Nancy Miorelli

 Sodium

Despite several studies looking into it, it appears that sodium doesn’t affect female reproductive success. If them males uptake a lot of sodium it doesn’t seem to make the females lay more eggs. Males of the African Fruit Feeding Butterflies had smaller sperm packets and took longer to mate when depleated of sodium  but again, the female’s reproductive success was unaffected.

There are some other ideas that more of the eggs hatch with sperm from males that ate more sodium. There’s another hyothesis that sodium helps with sperm motility but ants are known to puddle for salts also, and they don’t have sperm since the foragers are females. Another idea is that sodium helps amino acid uptake in the gut which helps the insects grow bigger and faster. There is supporting evidence in aphids  and skipper butterfies but Japanese beetles died with added sodium.

Aside from reproduction, it’s thought that maybe the male butterflies are more active and fly more and therefore need more salts to fuel their muscles. However, this doesn’t hold up consisetnly either and again ants puddle for salts and the foragers don’t have wings.

Nitrogen

Nitrogen is the  other thing the butterflies are probably looking for. We don’t know exactly what it’s doing, but we do know that we can trace nitrogren from when a male eats it to the eggs that the female in which he mated with lays. We also know that the cockroach Xestoblatta hamata actively feeds on bird poop (which is super rich in nitrogen) right before mating.

But basically, the sum of it is that we still don’t really know, although something to do with mating is probably most  of the answer. We also don’t know how their guts are suited to handle the uptake of these minerals but there’s a possibility that not all species of butterflies puddle on all types of surfaces.

Dangerous Business, Puddling

Butterflies  just sit on poop, carrion, and riverbanks for sometimes hours at a time. They’re relatively docile and can often be handled. So this begs the question, don’t predators eat them?

And this hasn’t been looked into much either. Butterflies often puddle in groups which seems to save them from bird attacks. Butterflies feeding on poop are especially docile and it could be that they’re temporarily inedible to predators.

There’s also some evidence of disease transmission as the butterflies that puddle on tears transmit diseases to their vertebrate hosts.

But basically we just don’t know and we need some more researchers.

If we puddle together in groups then we're less likely to be munched on by predators. PC: Nancy Miorelli

If we puddle together in groups then we’re less likely to be munched on by predators.
PC: Nancy Miorelli

TL;DR

Butterflies sit on poop, dead things, and riverbanks to absorb minerals like nitrogen and sodium. We’re not really sure why  they do this, but the limited evidence we have seems to suggest it’s important for mating in some way. Butterflies are able to puddle due to microsponges found at the tip of their tongue. We assume that there is some cost to puddling, but there aren’t many studies on the subject. Basically, if you like butterflies, and want to go into research  there’s a big gaping hole in the butterfly research waiting just for you!

Puddling swallowtails. PC: Nancy Miorelli

Puddling swallowtails.
PC: Nancy Miorelli

References:

  1. Krenn, HW. 2010. Feeding mechanisms of adult Lepidoptera: structure, function, and evolution of the mouthparts. Annual Review of Entomology 55: 307-327.
  2. Molleman F. 2009. Puddling: from natural history to understanding how it affects fitness. Entomologia Experimentalis et Applicata  134(2): 107-113
  3. Molleman F, Zwaan BJ, Brakefield PM. 2004. The effect of male sodium diet and mating history on female reproduction in the puddling squinting brush brown Bicyclus anynana (Lepidoptera). Behavioral Ecology Sociobiology 56: 404-411
  4. Monaenkova D, Lehnert MS, Andrukh T, Beard CE, Rubin B, et al. 2011. Butterfly proboscis: combining a drinking straw with a nanosponge facilitated diversification of feeding habits. Journal of the Royal Society Interface DOI: 10.1098/rsif.2011.0392

About Nancy

Entomologist, Science Communicator, and Crafter Twitter: @SciBugs
This entry was posted in Behavior, Ecology and tagged , , , , . Bookmark the permalink.

Discuss with Us

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s